11/28/15

How Bd has impacted Arizona’s amphibian species

A Frogs Are Green eco-interview with Nick Massimo and Evan Brus.

When was your organization founded? Please tell us a bit about its/your mission, goals… When did you first begin this important work?

Evan and I are both PhD students studying the disease dynamics of a deadly fungal pathogen that infects amphibians all over the world. The pathogen is Batrachochtrium dendrobatidis (Bd), and it causes the disease called chytridiomycosis. This disease was first noticed in the late 1980s and early 1990s as a wave of mass amphibian mortality events swept through Central America. Further research linked Bd to mass amphibian die offs and the extinction of multiple amphibian species all over the world.

Recent research has created a picture of how this pathogen may have originated in Brazil and spread to North America over 100 years ago. The oldest amphibian specimen that’s tested positive for Bd was in Illinois in 1888. Then, in the 1920s Bd was detected in California. Approximately 50 years passed before Bd was detected again in southern Mexico in 1972. The detection of Bd in Mexico was then tracked south into Central America where researchers first noticed the dramatic effects Bd could have on amphibian communities. The goal of our project is to better understand how Bd spread in the Americas, possibly passing through Arizona and to determine how this pathogen has impacted Arizona’s numerous amphibian species.

What is your educational background and what lead to wanting to specialize in this area and/or create your organization?

Nick Massimo

Nick MassimoI received a BS in biology and nutritional sciences and a minor in chemistry from the University of Arizona in 2011. After I graduated with my BS I spent two years working on numerous research projects studying species such as the Gila monster (Heloderma suspectum), desert tortoises (Gopherus agassizii), fungal endophytes (fungi that live inside plants), and several other herpertofauna species from the southwestern United States. I gained these experiences working with the University of Arizona, the Arizona Game and Fish Department and a not-for-profit group, Friends of Saguaro National Park. The combination of knowledge I gained from mycological and herpetological research in Arizona provided a great platform for me to pursue my PhD at Arizona State University studying how a fungal pathogen affects Arizona amphibians.

 

Evan Brus

Evan BrusI received my B.S in Zoology, French, and Environmental Studies from the University of Wisconsin Madison in 2011. During and after college, I worked in a social insect behavior lab studying the signals and cues that regulate nest construction in Polybia occidentalis, eventually traveling to Costa Rica to do research there. While studying for an ecology course, I happened upon the book Extinction in Our Times by my current advisor, James Collins. I was immediately intrigued by the story of amphibian declines, and I applied to do my PhD in his lab at ASU the following year.

 

Who would say has influenced you or your work?

Nick Massimo

I was inspired to pursue a career in biodiversity conservation by my early childhood experiences where I spent time with my family enjoying the great outdoors. Through these experiences I developed a deep appreciation of our beautifully complex natural world. The time I spent at the University of Arizona pursuing my BS continued to guide me to pursue a career in conservation due to some of my wonderful professors. Dr. Robichaux, Dr. Bonine and especially Dr. Arnold helped provide me with fantastic experiences and mentoring opportunities at the University of Arizona. Towards the end of my bachelor degree I received an internship with the Arizona Game and Fish Department. My advisor, David Grandmaison, introduced me to a wide array of projects focusing on conservation strategies for mammal, bird, reptile and amphibian species in Arizona.

After I graduated, I received a job with my most influential mentor, Dr. Betsy Arnold. During my time working with Dr. Arnold, I gained a large amount of experience on how to develop, conduct and conclude an intricate research project working with fungi that live inside of plants. Without the help and mentorship Dr. Arnold provided me, I would not be where I am today. Dr. Arnold has been my most influential mentor.

 

Evan Brus

Like many biologists and ecologists, I grew up with a deep appreciation for nature. I was lucky enough to live in a relatively rural area of Wisconsin surrounded by streams and forests, which I relished as a child. My early experiences exploring the outdoors informed my initial choice to study biology, but I credit several college professors for helping me refine my interests and encouraging my work. My undergraduate research mentor Dr. Robert Jeanne, as well as his grad students Ben Taylor and Teresa Schueller, taught me the culture of academia and the skills needed for data collection, analysis, and eventual publication. Without my experiences in this research group, I probably would not be a scientist today. My other major influence was Dr. Calvin DeWitt, who taught my introductory ecology course with fervent enthusiasm and took personal interest in all his students. I learned from him how to pursue my passions and how to visualize the myriad connections between humans and the natural world in everyday life. Currently, I draw inspiration from the broad community of scientists researching amphibian decline and chytridiomycosis, too many to name. The quantity and quality of research produced in our field is remarkable, and everyone is willing to help one another and collaborate. It’s a very welcoming and dedicated community.

 

Please share the details of your work: the medium, technique and discoveries.

Our project will analyze how Bd may have been introduced into Arizona and how its introduction could have shaped Arizona amphibian distributions. In order to investigate these questions amphibian specimens housed in natural history collections will be assessed for the presence of Bd. Our work will determine at what time were certain species infected with Bd in the state, and do these historic infections help us understand the dramatic decline of leopard frog species in Arizona.

testing Arizona frogs for Bd

Our work will rely on testing amphibian skin for the presence of Bd DNA. In order to do this, we will swab amphibian specimens stored in natural history. DNA extractions will then be performed following protocols used by other researchers who have recently completed similar projects analyzing museum specimens. After DNA extractions are complete, real time PCR will be used to determine if fungal pathogen DNA is present and if so, quantify the pathogen load on the specimen. Once all of this data is collected we will gain an understanding of the disease dynamics of Bd in Arizona. This information will help determine when this deadly pathogen arrived in Arizona, what species of amphibians it infected after it arrived across time, and a possible mechanism that brought it to the state (e.g. invasive species, the bait trade etc.). Finally, this information will be used to help establish management plans to mitigate the spread of this pathogen in amphibian populations that are currently infected with Bd and also provide critical information on how to keep Bd out of populations that are currently uninfected.

We have not started our project yet. Other researchers who have recently completed similar projects have discovered Bd on amphibians in natural history collections in the past. Their work has helped us form our hypotheses on how Bd may have entered Arizona as it spread south into Mexico and Central America. Based off of a previous project we know that Bd was found on a Chiricahua Leopard Frog in Arizona as early as 1972. We would like to conduct an extensive project to discover the very important disease dynamics of Bd in Arizona, that’s why we are trying to raise funds for our research with the Instrumentl crowdfunding campaign (https://www.instrumentl.com/campaigns/history-of-a-deadly-disease-in-arizona-amphibians/).

how Bd may have spread through the Americas

How Bd may have spread to Arizona

What has been the most exciting discovery?

As we reviewed recent research conducted by other scientists we were intrigued to learn that this fungal pathogen has a very complex history. Unraveling the history of this extremely deadly amphibian disease is made even more moving as amphibians are the most threatened Class of vertebrates on the planet (IUCN Red List Report 2015). This information paired with recent research indicates that amphibians are experiencing extraordinarily high extinction rates currently than they have in the past. We find this information extremely motivating, as we’d like to conserve amphibian biodiversity for the well being of ecosystems and for our future generations.

What are some challenges you have faced and how did you deal with them?

The biggest challenge we are currently facing is obtaining funding to conduct this important project. The scientific field as a whole has been experiencing a shortage of funds to carry out extremely important research projects, and our field is no different. To combat this issue, we are currently exploring new avenues to fund our research endeavors. This is where the crowdfunding campaign (https://www.instrumentl.com/campaigns/history-of-a-deadly-disease-in-arizona-amphibians/) comes into play. We are trying to reach out to our community more directly and get them engaged with our project. We’d like to communicate the challenge we, as scientists face, as well as the challenges amphibians are now incurring.

How do you reach your targeted audience?

Is it through your website, exhibitions, advertising or social media or another route? Which is most effective and why?

We are exploring a variety of options to engage our audience. We have been reaching out to our close colleagues, research and herpetology community directly through word of mouth and email to generate support for our project. Additionally, we have also used social media (Facebook and Twitter) to gain support for our research project. Both of these avenues have provided support for our project but we’d like to reach more people. To do this, we are contacting people and groups located within Arizona to convey that this project will directly impact the amphibian biodiversity of our state and in other parts of the world.

How do you keep the audience engaged over time?

We’ve created a Facebook group (https://www.facebook.com/groups/905234229573944/) to keep our audience up to date on the progression of our research project as it’s being conducted. This will keep our audience up to date on the current status of our project and the exciting discoveries we’re making. In addition to our Facebook group, we are also planning to present the results of our study in a scientific journal article and present this information to our community in a series of talks.

What can we do to inspire the next generation to want to help the environment and the wildlife?

The information we gain from completing this study will directly lend to the construction of effective amphibian biodiversity conservation strategies. Without the help from our next generation we are at risk of losing much of the amazing amphibian biodiversity on our planet. The Frogs Are Green organization could be a wonderful partner in helping us to spread our project to its supporters. We feel that this organization can help reach a younger and broader audience than we’ve been able to reach thus far. In order for the meaningfulness of our research to be effective we need our next generations to know about the wonderful and beautiful amphibian biodiversity our planet has to offer.

What can people do to help? Donate and/or share to your cause/work?

We would be most appreciative if individuals would pledge to share our campaign with friends and colleagues using social media. We hope to reach as many people as possible because we feel the current state of our planet’s amphibian biodiversity is at a critical point. As a community we need to act now to preserve these species to maintain healthy ecosystems and for future generations. Sharing our campaign would help us reach our goal however we would be extremely thankful if people would also donate to our project so we can conduct this extremely important research.

08/3/15

Will the Fungus Killing Salamanders Come to the US?

There is nothing more important right now to the survival of Salamanders than the United States stopping the importation of them from Europe and Asia.

From the New York Times article, “Importing Both Salamanders and Their Potential Destruction.”

“You would think there would be something in place,” said Vance T. Vredenburg, a biologist at San Francisco State University. “We really need a government agency at some level to take action and do something.”

…The pet trade brings huge numbers of salamanders into the United States. Fish and Wildlife Service records show that about 780,000 salamanders were imported from 2010 to 2014. Dr. Vredenburg and his colleagues found that 98 percent of the imported animals were native to Asia.

We have been watching Chytrid Fungus, or Batrachochytrium dendrobatidis (Bd for short) wipe out many species of frogs. Humans have helped spread this disease around the world and now we have an opportunity, if we act quickly to help the salamanders.

We’re worried about the Asian fungus that causes the disease “Bsal” which has already reached Europe, wiping out 96 percent of fire salamanders in the Netherlands. Now, researchers have determined that the fungus will spread like wildfire if it reaches North America, and they’re calling for an immediate ban on all salamander imports.

Fire Salamander from Wikipedia Commons

Fire Salamander from Wikipedia Commons.

Below are a series of articles on this topic from the past week:

NPR >> Scientists Urge Ban On Salamander Imports To U.S. To Keep Fungus At Bay

 

UC Berkeley >> Scientists urge ban on salamander imports to fend off new fungus

 

The Verge >> North America’s salamanders threatened by bloody skin disease

 

Tech Times >> Bsal Fungus Can Wipe Out U.S. Salamander Population: What To Know

02/27/15

Chytrid Fungus is Found in Madagascar

The entire amphibian class is currently afflicted by a global pandemic that is accelerating extinction at an alarming rate. Until now, a few islands like Madagascar were thought not to have been affected. However, an analysis of the latest series of tests shows that the chytrid fungus also poses a threat to amphibians in Madagascar. “This is sad news for herpetologists around the world,” says Dr. Dirk Schmeller of the UFZ, who was involved in analyzing the samples and has, together with Elodie Courtois, detected Bd in samples from Madagascar collected in 2010. “Firstly, it means that an island that is home to a particularly high number of amphibian species is now at risk. Several hundred species live only on this island. And, secondly, if the pathogen has managed to reach such a secluded island, it can and will occur everywhere.”

frog by Miguel Vences / TU Braunschweig

IMAGE: Chytrid fungus was proved on Platypelis pollicaris from Ranomafana. view more
Credit: Miguel Vences / TU Braunschweig

Prof. Miguel Vences from TU Braunschweig adds, “The chytrid fungus was found in all four families of the indigenous Madagascan frogs, which means it has the potential to infect diverse species. This is a shock!” The study also shows that the disease affects amphibians at medium to high altitudes, which ties in with observations from other parts of the world, where the effects of the amphibian epidemic have been felt primarily in the mountains.

“Luckily, there have not yet been any dramatic declines in amphibian populations in Madagascar,” Dirk Schmeller reports. “However, the pathogen appears to be more widespread in some places than others. Madagascar may have several strains of the pathogen, maybe even the global, hypervirulent strain. This shows how important it is to be able to isolate the pathogen and analyze it genetically, which is something we haven’t yet succeeded in doing.” At the same time, the researchers recommend continuing with the monitoring program across the entire country to observe the spread of the disease. The scientists also suggest setting up extra breeding stations for key species, in addition to the two centers already being built, to act as arks, so that enough amphibians could be bred to recolonize the habitats in a crisis. “We are also hopeful that we may be able to suppress the growth of the Bd pathogen with the help of skin bacteria,” says Miguel Vences. “It might then be possible to use these bacteria as a kind of probiotic skin ointment in the future.” A high diversity of microbial communities in the water could also reduce the potential for infection, according to earlier investigations conducted by Dirk S. Schmeller, Frank Pasmans, and their teams (published in Current Biology).

The outbreak of amphibian chytridiomycosis in Madagascar puts an additional seven per cent of the world’s amphibian species at risk, according to figures from the Amphibian Survival Alliance (ASA). “The decline in Madagascan amphibians is not just a concern for herpetologists and frog researchers,” says Dr. Franco Andreone, “It would be a great loss for the entire world.”
 
Guest post by Dr. Dirk Schmeller
Originally published:
http://www.eurekalert.org/pub_releases/2015-02/hcfe-acf022615.php

02/8/15

Frogs and Water Quality

Yesterday, in class with the Cloud Institute and Sustainable Jersey (NJ Learns), each student got to briefly share their project, so I was describing the Frogs Are Green mission and projects on the agenda for 2015.

My teacher Jaimie P. Cloud, challenged me with this question. “Much of what Frogs Are Green is about is bringing awareness to the public, but is there a change I want in the public’s behavior?”

It made me think about one specific area that we can focus on which would help frogs and amphibians as well as other wildlife and humans, and that is water quality.

We know that there are many issues, such as, pollution and oil spills, pesticides and other toxins, pharmaceuticals, and these aren’t just affecting wildlife, but us as well. It’s humans that are creating many of these problems, so it’s obvious that we must be the ones to correct it.

testing water quality

I did a search on Google for Frogs and Water Quality and was surprised to learn there is currently no standard for water quality as it applies to frogs and amphibians:
“The factors thought to be contributing to declines in frog populations include habitat loss, introduction of exotic species, overexploitation, disease, climate change, and decreasing water quality. With respect to water quality, agroecosystems use 80-90% of the water resources in the western United States, frequently resulting in highly eutrophic conditions. Recent investigations suggest that these eutrophic conditions (elevated pH, water temperature, and un-ionized ammonia) may be associated with frog embryo mortality or malformations. However, water quality criteria for frogs and other amphibians do not currently exist. Here, we briefly review data that support the need to develop water quality parameters for frogs in agroecosystems and other habitats.”
(from this website: PMC1519110/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1519110/)

What this tells me is that currently the water quality is tested to make sure it’s safe for humans, but there are no regulations in place for it to be safe for wildlife? Doesn’t it matter that it’s healthy for all life?

I’ll be reading much more on this issue and would like to invite you to share your thoughts or links to additional documentation you may have found.

Here’s a video I’d like to share because it shows just how important it is to educate the young with hands-on education from Marie Hartford, Science, 5th Grade Teacher and her students in Redmond, WA on the Teaching Channel about “Measuring the pH as it Relates to Water Quality.”

08/1/14

Tadpoles in Maine Pond Die from Ranavirus

An estimated 200,000 Wood Frog (Lithobates sylvaticus) Tadpoles in a local pond in Maine died within a few days (in 2013) from most-likely a ranavirus. A paper was recently published in Herpetological Review that is co-authored by Nathaniel Wheelwright, a biologist at Bowdoin College, along with the University of Tennessee.

“The dead tadpoles had signs of hemorrhaging in their legs and around their throats, and many had skin that was sloughing off their bodies”, Wheelwright said.

Wheelwright has monitored the pond for close to 30 years and said it was quite surprising. Usually there are 50-100,000 tadpoles and only 1000 may live to become adult frogs. But for all to die, very unusual. They did notice there were no leeches present.

There are other species who can carry the ranavirus, such as green frogs, bullfrogs, painted turtles and spotted salamanders* (*who were found in the pond and showed signs of suffering from the virus also.)

Here’s a video from Bowdoin College with biology professor, Nat Wheelwright talking about the tadpoles:

The good news is that this past Spring showed healthy tadpoles and frogs and no sign of any issues.

Do you think this was caused by pesticides and/or climate change? Too many tadpoles crowded together?
___________________________________________________________________

The differences between Chyrid Fungus and Ranavirus are:

Chyrid is a fungus and infects individual frogs who suffer damage to their skin. Once infected it impairs respiration and the frog dies. It has been devastating amphibian populations around the world.

Ranaviruses infect insects, fish, amphibians and turtles and infect larvae or recently metamorphosed individuals.

10/3/13

Protecting the Unique Frogs of Andasibe, Madagascar

Devin EdmondsGuest post by
Devin Edmonds,
Amphibian Conservation Director, Association Mitsinjo

It doesn’t get much better than a going into the forests of Andasibe at night.  There are leaf-tailed geckos, mouse lemurs, sleeping chameleons, and frogs. Lots of frogs. More than 100 different species, in fact, which have been identified in the surrounding forests. This is more than a third of all described frog species on the island.

Most of these frogs are nocturnal, but a few are also active during the day, like the Critically Endangered golden mantella (Mantella aurantiaca), which is only found in a small area near Andasibe in east-central Madagascar.

The conservation organization Mitsinjo works in the area around Andasibe, and is composed of 53 members of the community.

Our activities include:

  • Habitat management
  • Research
  • Nature-based tourism
  • Reforestation
  • Captive breeding
  • Environmental education

A group of our projects are targeted specifically at monitoring and addressing the threats our unique local frog species face. This includes the development of Madagascar’s first biosecure captive breeding facility capable of establishing assurance survival colonies of threatened amphibians.

Additionally, we conduct surveys to monitoring for declines and population changes. This activity compliments participation in a nation-wide early detection plan for the chytrid fungus Bd, the devastating pathogen contributing to alarming amphibian extinctions around the world. Fortunately, so far we have not detected Bd in Andasibe and reports elsewhere in Madagascar remain highly doubtful and unconfirmed.

Recently, Mitsinjo joined forces with the NGO Madagasikara Voakajy to contribute to the national conservation strategy for the golden mantella. Each month, we monitor three breeding sites at Torotorofotsy Wetland. This area is under tremendous pressure from artisanal gold mining, slash-and-burn agriculture, and charcoal production. Our habitat patrols help to ensure the breeding sites for this highly threatened amphibian remain intact.

To learn more about our organization like us on Facebook (www.facebook.com/Mitsinjo) and visit our website www.mitsinjo.org. For more information on amphibian conservation in Madagascar see www.sahonagasy.org
 

Andasibe Village

Andasibe Village, Madagascar

boophis luteus in amplexus

boophis luteus in amplexus

Captive breeding facility - Mitsinjo

Captive breeding facility - Mitsinjo

Captive breeding facility inside view - Mitsinjo

Captive breeding facility inside view - Mitsinjo

Mantella aurantiaca breeding site patrol at Torotorofotsy Wetland

Mantella aurantiaca breeding site patrol at Torotorofotsy Wetland

Mitsinjo swabs captive frogs for Bd

Mitsinjo swabs captive frogs for Bd

Mantidactylus species near Andasibe

Mantidactylus species near Andasibe

Mantella aurantiaca of Mitsinjo

Mantella aurantiaca of Mitsinjo

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