05/2/12

Chemical Pollution in Your Backyard: Researching the Effects of Endocrine Disruptors in Suburbia

We were so pleased to receive this guest post by Geoffrey Giller, who is a Master’s of Environmental Science candidate, 2013, at the Yale School of Forestry and Environmental Sciences. At Frogs Are Green, we have explored the issue of endocrine disruptors and their possible effects on frogs, as well as on humans. We look forward to learning the results of his research.

Geoffrey Giller environmental sciences Yale school

Geoff Giller readying nets for sampling at a golf course (Photo credit: Susan Bolden)

Frogs are threatened globally by a host of stressors, from habitat loss to climate change to infectious diseases. One threat receiving increased scientific attention is endocrine-disrupting chemicals (EDCs). These chemicals interfere with normal hormonal function. In 2002, Dr. Tyrone Hayes published a paper in Nature detailing the feminizing effects of the widely-used pesticide atrazine on male African clawed frogs. Since then, there has been increasing research showing similar effects of other EDCs on frogs and fish. There is also more and more evidence that endocrine disruptors are widely prevalent pollutants in the water bodies of the United States.

As a Master’s student at Yale’s School of Forestry and Environmental Studies, I want to further investigate the presence and effects of these EDCs. My advisor, Dr. David Skelly, recently published a paper showing that green frog hermaphrodites are most prevalent in suburban areas. While most research since Dr. Hayes’ paper has focused on agricultural areas, Dr. Skelly’s work indicated that there may be a significant source of EDCs causing these high rates of hermaphroditism (as demonstrated by the presence of egg cells in the gonads of male green frogs) in suburban areas.

photomicrographic frog cells

Testicular oocytes (egg cells in male gonads) from Skelly et al. 2010

This topic has implications both for frog conservation, as such sexual deformities likely inhibit frogs’ abilities to reproduce, and for human health, since suburban areas are much more densely populated than agricultural ones. But while the presence of these deformities has been confirmed, their cause has not. Dr. Skelly has hypothesized that the cause may be certain EDCs called synthetic estrogenic compounds; these chemicals are found in medications such as birth control pills as well as in some cosmetic products. The source of these chemicals would likely be septic tanks that leach chemicals into the groundwater; this contaminated groundwater then flows into nearby ponds, streams, and wetlands where frogs breed and live.

I am working with my fellow Master’s student, Max Lambert, to identify exactly what chemicals are present in the groundwater and pond water in suburban areas and what effect these chemicals are having on frogs. In collaboration with scientists at USGS, we will be installing sampling devices at 9 suburban sites, as well as three agricultural and three forested sites as comparisons. We will sample the groundwater and the pond water for a large range of chemicals including medications, synthetic estrogens, pesticides, and a host of other man-made compounds. This study will provide a list of the chemicals that may be responsible for the deformities in the frogs that we are observing.

Small Frog size of fingernail

Max Lambert with a peeper (Photo credit: Hannah Erin Bement)

In addition to the water testing, we will be surveying three types of frogs—American toads, gray tree frogs, and pickerel frogs—for similar sexual deformities. By combining the data from our water testing with the rates of deformities in frogs at these same ponds, we will be able to say which chemicals are likely causing deformities, and which are not. This information will be crucial for the future regulation of these chemicals.

The main stumbling block of this project is the cost. EDCs can have significant biological effects when present at very low concentrations; however, doing water testing for such low concentrations of chemicals requires highly specialized equipment. While some of our costs are covered by our collaboration with USGS, we still need to raise some funds. For more information on our project, and for a chance to help us out with this research, please go here: http://www.petridish.org/projects/estrogens-in-your-backyard-the-chemical-ecology-of-suburbia. This website allows researchers to raise necessary resources through “crowdfunding,” or multiple small donations from a large number of people. We are close to our target funding amount, but could use a little more help. Take a look at our page (as well as the various rewards for different donation amounts), and we would be incredibly grateful for your support!

04/28/12

Save the Frogs Day 2012!

Today is Save the Frogs Day, the world’s largest day of amphibian education and conservation action, organized by Save the Frogs, an organization dedicated to protecting the world’s amphibian species, founded by ecologist Dr. Kerry Kriger.

Funds raised in the many Save the Frogs Day events go in part for grants to help amphibian projects in developing countries that have high rates of deforestation, including Nepal, Madagascar, and Liberia. Without funds from Save the Frogs, these projects might not have been possible.

At Frogs Are Green, we would like to encourage you to check out the events that are going on today around the world, and also to urge you to do something, even something small, to help out. Educating just one person about the plight of amphibians will help.

One-third of all amphibians are at risk of extinction and we should be concerned. After all, we humans also share this planet with amphibians, a planet that seems to have become too unhealthy for an entire group of animals. By saving frogs, we also save ourselves.

Please see the Save the Frogs site for lots of suggestions for getting involved on Save the Frogs Day—and everyday.

Here’s a cool t-shirt you can buy over at Save the Frogs:

04/17/12

Earth Day 2012: Plant a Tree!

This Earth Day, our theme is simple: Plant a tree. We were inspired by a recent op-ed by Jim Robbins in the New York Times: “Why Trees Matter. “And we were also inspired by the beauty of the springtime trees around us.

At Stevens Institute, Hoboken. Photo by Mary Jo Rhodes

In the NY Times piece, Robbins explains how trees are at the forefront of climate change. Hot, drier weather is stressing, and often killing, trees worldwide. The examples he cites include some of North America’s most ancient trees, the alpine bristlecone forests, which are falling victim to a voracious beetle and an Asian fungus. Prolonged droughts have killed more than five million urban shade trees last year. In the Amazon, two severe droughts have killed billions more.

And yet, trees perform essential functions that we don’t always appreciate. Here are a few Robbins highlights:

  • Through photosynthesis, trees turn sunlight into food for insects, wildlife, and people (apple or pear anyone?), as well as create wood for fuel, furniture, and homes. Trees contribute to our emotional well being by providing beauty in our surroundings and much needed shade.
  • When tree leaves decompose, they leach acids into the ocean that help fertilize plankton. When plankton thrive, so does the rest of the food chain. Fishermen have replanted forests along coasts and rivers to successfully bring back depleted fish and oyster stocks.
  • Trees release beneficial chemicals that seems to help regulate the climate; others are anti-bacterial, anti-fungal and anti-viral. Aspirin’s active ingredient, for example, comes from willows.
  • Trees are the planet’s heat shield. They keep the concrete and asphalt of cities and suburbs 10 or more degrees cooler and protect our skin from the sun’s harsh UV rays.

Plant a Tree

I live in one of the most densely populated cities in the U.S. Some years ago, I planted a small tree (about a foot high, purchased from a nursery) in our backyard with my sons. The tree is now about 20 feet high and we enjoy watching the leaves change each fall and the birds hanging out on the branches.

No matter where you live, you can plant a tree.

Even if you live in an apartment and don’t have a back yard, you might be able to find a tree-planting initiative in your city. New York City, for example, has an initiative called MillionTreesNYC, in which volunteers plant trees or adopt trees and care for them after they’re planted.

View of Empire State Building, at Stevens Institute of Technology, Hoboken. Photo by Mary Jo Rhodes.

And, of course, our amphibian friends need trees, especially those arboreal frogs in the Hylidae family, many of which live in tropical and temperate forests.

For more information (including information about where to buy tree seedlings), see the Arbor Day Foundation.

Happy Earth Day!

04/4/12

How Does a Toad Cross the Road? With a lot of Help from their Friends

As the days are getting longer and the sun is getting warmer in the Northern Hemisphere, a young male toad’s thoughts turn to love – it’s mating time for amphibians. Unfortunately this springtime ritual is often fraught with danger for frogs and toads. As we were looking for stories for this week’s post, we noticed a lot of stories about how people in Britain and the U.S. are helping toads make this important but dangerous journey. Here are a few:

Great Britain

Blades of glass rustle as hundreds — if not thousands — of toads spring forth from hibernation, each one a bloated, wart-ridden, writhing mass of arms of legs. Then, wide-eyed and kamikaze-like, they fall from the high kerb [curb] before making a desperate dash across the road, determined to reach their hereditary breeding ground. (As reported by  in the Henley Standard, Henley-on-Thames, England)

Unfortunately the toads in Henley must contend with a human-created challenge — rush-hour. Local toad patrols, however, are helping the toads cross the road. This year they’ve saved 7,500 toads from road death.  The toad patrols can’t stop traffic, but motorists slow down when they see their toad crossing signs and their high-visibility jackets.

Henley Wildlife Group Toad Patrol, courtesy of the Henley Standard, Henley-on-Thames, England

Volunteers spent every night of the past month helping toads by carrying them in buckets. The Toad Patrol happens every year and saves about 80 per cent of the migrating population of toads.

The BBC reports that in Northhampshire, England, hundreds of toads make a yearly one-mile journey from a woodland across a number of roads to an area that is now a housing development in the village of St. Crispins, to the south of Northampton.

Many toads are squashed by cars and dozens more fall into drains as they try to get back to where they were born. But those that do make it alive will find that the pond has been replaced by buildings. Donna Robins, a toad patroller, was quoted in the article as saying: “My house is on the woodland where they used to live, I feel responsible. I see them getting killed every night on the road.”

In Edinburgh, Scotland, the Historic Scotland Rangers are helping out with the toad annual ‘Watch Out, Toads About,‘ which helps toads migrate. Early every morning Rangers carry out patrols to check the nooks, crannies, and drains in Holyrood Park to make sure each toad gets to its destination safely.

In winter the toads live around Arthur’s Seat [the beautiful small mountain right outside the city] and Dunsapie Loch. In order to get to where they spawn, they must cross a public road with high curbs. When the toads are tired and hungry they can become disoriented and may be unable to make that last leap to safety. Volunteers are needed to help the roads cross the road safely.

United States

As reported in the Roxborough (PA) Patch.com, frogs and toads are on the move and in an annual tradition,the Schuylkill Center for Environmental Education has helped them along.

photo courtesy The Schuylkill Environmental Center

The environmental group coordinates volunteer shifts to aid the toads crossing roads. There’s lots of information about volunteering on their site. After the toads breed, the toadlets, about the size of a fingernail, make their move from May 15 to June 30 and hop back to the woods, so volunteers can pitch in later, too.

Recently, according to the Provincetown (MA) Journal, the Cape Cod National Seashore issue an announcement that beginning this spring and continuing through October, certain local roads will  be closed at times, particularly on rainy nights, to protect the spadefoot toad, which is listed by the state as a threatened species.

These unusual toads, one of 12 species of amphibians found within the Seashore, use shallow, temporary ponds for breeding, and the surrounding uplands to feed. They migrate to and from these ponds on rainy nights, especially when the water table is high and temperatures are above 48 degrees.

The toads must cross paved roads, and during such crossings many of these well-camouflaged creatures are unfortunately killed by moving vehicles. Thus there is a need for carefully timed, strategic, temporary detours.

The Province Lands area of Cape Cod is one of the last strongholds for the eastern spadefoot toad in Massachusetts, and the public’s cooperation — and forbearance — is urged in the effort to ensure their survival.

As reported in the article [and mentioned as a difficult issue in some of the other articles cite above], sometimes the public isn’t very enthusiastic about the measures to help amphibians:

A related challenge to protecting these elusive creatures is raising the public’s consciousness about their importance environmentally. By their very nature, spadefoot toads do not attract the throngs of admirers that, say, whales do. Because of their size, habits and habitat, the toads are hard to find and quantify, and are challenging to study. Further, by most standards, they are not very glamorous — except perhaps to herpetologists.

While their tiny, almost invisible close “cousins” the spring peepers can claim status for their joyful, lilting songs that herald the arrival of spring on the Cape, the pained dyspeptic croaking of a spadefoot induces no similar elation. However, in the larger environmental scheme of things they are important marvels of adaptation….

The public can listen in on recordings of frog and toad calls, as well as discussions on amphibian ecology, via links to podcasts and videos on the Seashore’s website. At Frog are Green, we’d like to wish these toads “safe travels” and applaud all  the Toad Patrols in the US and the UK who are helping them along on their journeys.

03/19/12

If You Can Make It Here: New frog species discovered in NYC

Last week’s discovery of a new frog species in New York City was one of our favorite recent amphibian news stories. The story was picked up by newspapers both across the country and worldwide, from the BBC to the News Pakistan. We especially liked the story, not only because we are both native New Yorkers, born within an hour’s drive of where this frog was discovered, but also because it was discovered by a scientist from New Jersey (our adopted state.)

So here’s the story, as reported by the New York Times and New Jersey Newsroom.com:

While doing research in Staten Island (one of New York City’s boroughs) in 2009, Jeremy A. Feinberg, a doctoral candidate in ecology and evolution at Rutgers University, heard an unusual frog call.  Instead of the “long snore” or “rapid chuckle” he would normally expect from a  leopard frog, he heard instead a short, repetitive croak. Feinberg suspected this frog might be a new species. He teamed up with Cathy Newman, a geneticist completing a master’s degree in genetics at the University of Alabama, to test the frog’s DNA.

Jeremy Feinberg

Newman compared this frog’s DNA with the DNA of southern and northern leopard frogs, which range widely north and south of New York City. These frogs look quite similar to each other, but the results indicated that this frog’s lineage was genetically distinct.

Newly discovered leopard frog in NYC. Photo by Brian Curry, Rutgers University

Feinberg believes this leopard frog once inhabited Manhattan and the other boroughs. He has found specimens in the Meadowlands and the Great Swamp National Wildlife Refuge in New Jersey, as well as in Putnam and Orange Counties in New York. Some frogs were also collected in central Connecticut.

What’s unusual about this finding is that new frog species are usually found in the remote rainforests of Indonesia and similar places, and not within the shadow of one of the world’s most densely populated urban areas.

The New York Times has asked readers to come up with a name for this new frog. They have listed some attributes of this frog to give you inspiration for a name, including the fact that the geographic center of the frog’s range is Yankee Stadium in the Bronx.

How about The Green Bomber? After all, there are Yankee fans all over the tri-state area.

More information about the discovery:

The findings are to be published in an issue of the journal Molecular Phylogenetics and Evolution, but are currently available online. Much of the genetic analysis was performed in Professor  H. Bradley Shaffer’s laboratory at the University of California at Davis, where he worked until recently.

Photo of Jeremy Feinberg, courtesy of New Jersey Newsroom.com

03/11/12

Become a FrogWatch USA Volunteer: Listen to your Local Frogs

In the U.S., frogs and toads are beginning to wake up from their winter hibernation and soon we’ll be hearing the calls of spring as the amphibian breeding season begins. This a great time to become a Frog Watch USA volunteer, where you will make a commitment to monitor a local site for 3 minutes at least twice a week throughout the breeding season.

You don’t have to be an expert to become a volunteer, but you might find it helpful to attend a Frog Watch training session hosted by zoos, aquariums, and conservation organizations nationwide. Here’s a list of the upcoming training sessions:

Connecticut
Yale Peabody Museum of Natural History
March 16, 2012; 6:00-8:00 pm
March 20, 2012; 6:00-8:00 pm

Florida
Brevard Zoo
April 11, 2012; 4:30-8:30 pm
April 14, 2012; 4:30-8:30 pm
May 23, 2012; 4:30-8:30 pm
June 20, 2012; 4:30-8:30 pm
July 25, 2012; 4:30-8:30 pm
August 22, 2012; 4:30-8:30 pm
August 25, 2012; 4:30-8:30 pm

Santa Fe College Teaching Zoo, Gainesville, FL
March 17, 2012

Tampa’s Lowry Park Zoo
April 5, 2012; 6:30-8:30 pm (volunteer training)
May 3, 2012; 6:30-8:30 pm (call identification and certification)
June 7, 2012; 6:30-8:30 pm (volunteer training)
July 5, 2012; 6:30-8:30 pm (call identification and certification)
August 2, 2012; 6:30-8:30 pm (end-of-season wrap up/pot luck)

Indiana
Fort Wayne Children’s Zoo
March 13, 2012; 5:30-9:30 pm
March 17, 2012; 1:00-5:30 pm

Monroe County Parks and Rec
March 22, 2012; 6:00-9:00 pm

Michigan
Detroit Zoo
March 11, 2012; 1:00-4:00 pm
March 18, 2012; 1:00-4:00 pm

Missouri
Saint Louis Zoo
March 24, 2012; 10:00 am-12:30 pm
March 28, 2012; 7:00-9:00 pm (certification)

New Jersey
Jenkinson’s Aquarium
March 21, 2012; 6:00-8:00 pm

Rhode Island
Roger Williams Park Zoo
March 24, 2012; 10:00am-12:00 pm
April 12, 2012; 6:00-8:00 pm

Tennessee
Chatanooga Zoo
March 31, 2012

Utah
Utah’s Hogle Zoo
March 17, 2012; 2:00-4:00pm

Virginia
Virginia Zoo, March 18, 2012; 5:00pm

At a recent training session at the Lynchburg (VA) Public Library, for example, volunteers listened to the calls and then tried to connect them to a recognizable sound. Here’s one of the frog calls these volunteers tried to identify. Does the call of this Pickerel frog sound to you like a squeaky door – or like a snore?

More information:

The FrogWatch site includes a Frogs and Toads by State list and a link to the U.S. Geological Survey Frog Quiz of frog calls.