11/28/15

How Bd has impacted Arizona’s amphibian species

A Frogs Are Green eco-interview with Nick Massimo and Evan Brus.

When was your organization founded? Please tell us a bit about its/your mission, goals… When did you first begin this important work?

Evan and I are both PhD students studying the disease dynamics of a deadly fungal pathogen that infects amphibians all over the world. The pathogen is Batrachochtrium dendrobatidis (Bd), and it causes the disease called chytridiomycosis. This disease was first noticed in the late 1980s and early 1990s as a wave of mass amphibian mortality events swept through Central America. Further research linked Bd to mass amphibian die offs and the extinction of multiple amphibian species all over the world.

Recent research has created a picture of how this pathogen may have originated in Brazil and spread to North America over 100 years ago. The oldest amphibian specimen that’s tested positive for Bd was in Illinois in 1888. Then, in the 1920s Bd was detected in California. Approximately 50 years passed before Bd was detected again in southern Mexico in 1972. The detection of Bd in Mexico was then tracked south into Central America where researchers first noticed the dramatic effects Bd could have on amphibian communities. The goal of our project is to better understand how Bd spread in the Americas, possibly passing through Arizona and to determine how this pathogen has impacted Arizona’s numerous amphibian species.

What is your educational background and what lead to wanting to specialize in this area and/or create your organization?

Nick Massimo

I received a BS in biology and nutritional sciences and a minor in chemistry from the University of Arizona in 2011. After I graduated with my BS I spent two years working on numerous research projects studying species such as the Gila monster (Heloderma suspectum), desert tortoises (Gopherus agassizii), fungal endophytes (fungi that live inside plants), and several other herpertofauna species from the southwestern United States. I gained these experiences working with the University of Arizona, the Arizona Game and Fish Department and a not-for-profit group, Friends of Saguaro National Park. The combination of knowledge I gained from mycological and herpetological research in Arizona provided a great platform for me to pursue my PhD at Arizona State University studying how a fungal pathogen affects Arizona amphibians.

Evan Brus

I received my B.S in Zoology, French, and Environmental Studies from the University of Wisconsin Madison in 2011. During and after college, I worked in a social insect behavior lab studying the signals and cues that regulate nest construction in Polybia occidentalis, eventually traveling to Costa Rica to do research there. While studying for an ecology course, I happened upon the book Extinction in Our Times by my current advisor, James Collins. I was immediately intrigued by the story of amphibian declines, and I applied to do my PhD in his lab at ASU the following year.

Who would say has influenced you or your work?

Nick Massimo

I was inspired to pursue a career in biodiversity conservation by my early childhood experiences where I spent time with my family enjoying the great outdoors. Through these experiences I developed a deep appreciation of our beautifully complex natural world. The time I spent at the University of Arizona pursuing my BS continued to guide me to pursue a career in conservation due to some of my wonderful professors. Dr. Robichaux, Dr. Bonine and especially Dr. Arnold helped provide me with fantastic experiences and mentoring opportunities at the University of Arizona. Towards the end of my bachelor degree I received an internship with the Arizona Game and Fish Department. My advisor, David Grandmaison, introduced me to a wide array of projects focusing on conservation strategies for mammal, bird, reptile and amphibian species in Arizona.

After I graduated, I received a job with my most influential mentor, Dr. Betsy Arnold. During my time working with Dr. Arnold, I gained a large amount of experience on how to develop, conduct and conclude an intricate research project working with fungi that live inside of plants. Without the help and mentorship Dr. Arnold provided me, I would not be where I am today. Dr. Arnold has been my most influential mentor.

Evan Brus

Like many biologists and ecologists, I grew up with a deep appreciation for nature. I was lucky enough to live in a relatively rural area of Wisconsin surrounded by streams and forests, which I relished as a child. My early experiences exploring the outdoors informed my initial choice to study biology, but I credit several college professors for helping me refine my interests and encouraging my work. My undergraduate research mentor Dr. Robert Jeanne, as well as his grad students Ben Taylor and Teresa Schueller, taught me the culture of academia and the skills needed for data collection, analysis, and eventual publication. Without my experiences in this research group, I probably would not be a scientist today. My other major influence was Dr. Calvin DeWitt, who taught my introductory ecology course with fervent enthusiasm and took personal interest in all his students. I learned from him how to pursue my passions and how to visualize the myriad connections between humans and the natural world in everyday life. Currently, I draw inspiration from the broad community of scientists researching amphibian decline and chytridiomycosis, too many to name. The quantity and quality of research produced in our field is remarkable, and everyone is willing to help one another and collaborate. It’s a very welcoming and dedicated community.

Please share the details of your work: the medium, technique and discoveries.

Our project will analyze how Bd may have been introduced into Arizona and how its introduction could have shaped Arizona amphibian distributions. In order to investigate these questions amphibian specimens housed in natural history collections will be assessed for the presence of Bd. Our work will determine at what time were certain species infected with Bd in the state, and do these historic infections help us understand the dramatic decline of leopard frog species in Arizona.

Our work will rely on testing amphibian skin for the presence of Bd DNA. In order to do this, we will swab amphibian specimens stored in natural history. DNA extractions will then be performed following protocols used by other researchers who have recently completed similar projects analyzing museum specimens. After DNA extractions are complete, real time PCR will be used to determine if fungal pathogen DNA is present and if so, quantify the pathogen load on the specimen. Once all of this data is collected we will gain an understanding of the disease dynamics of Bd in Arizona. This information will help determine when this deadly pathogen arrived in Arizona, what species of amphibians it infected after it arrived across time, and a possible mechanism that brought it to the state (e.g. invasive species, the bait trade etc.). Finally, this information will be used to help establish management plans to mitigate the spread of this pathogen in amphibian populations that are currently infected with Bd and also provide critical information on how to keep Bd out of populations that are currently uninfected.

We have not started our project yet. Other researchers who have recently completed similar projects have discovered Bd on amphibians in natural history collections in the past. Their work has helped us form our hypotheses on how Bd may have entered Arizona as it spread south into Mexico and Central America. Based off of a previous project we know that Bd was found on a Chiricahua Leopard Frog in Arizona as early as 1972. We would like to conduct an extensive project to discover the very important disease dynamics of Bd in Arizona, that’s why we are trying to raise funds for our research with the Instrumentl crowdfunding campaign (https://www.instrumentl.com/campaigns/history-of-a-deadly-disease-in-arizona-amphibians/).

What has been the most exciting discovery?

As we reviewed recent research conducted by other scientists we were intrigued to learn that this fungal pathogen has a very complex history. Unraveling the history of this extremely deadly amphibian disease is made even more moving as amphibians are the most threatened Class of vertebrates on the planet (IUCN Red List Report 2015). This information paired with recent research indicates that amphibians are experiencing extraordinarily high extinction rates currently than they have in the past. We find this information extremely motivating, as we’d like to conserve amphibian biodiversity for the well being of ecosystems and for our future generations.

What are some challenges you have faced and how did you deal with them?

The biggest challenge we are currently facing is obtaining funding to conduct this important project. The scientific field as a whole has been experiencing a shortage of funds to carry out extremely important research projects, and our field is no different. To combat this issue, we are currently exploring new avenues to fund our research endeavors. This is where the crowdfunding campaign (https://www.instrumentl.com/campaigns/history-of-a-deadly-disease-in-arizona-amphibians/) comes into play. We are trying to reach out to our community more directly and get them engaged with our project. We’d like to communicate the challenge we, as scientists face, as well as the challenges amphibians are now incurring.

How do you reach your targeted audience?

Is it through your website, exhibitions, advertising or social media or another route? Which is most effective and why?

We are exploring a variety of options to engage our audience. We have been reaching out to our close colleagues, research and herpetology community directly through word of mouth and email to generate support for our project. Additionally, we have also used social media (Facebook and Twitter) to gain support for our research project. Both of these avenues have provided support for our project but we’d like to reach more people. To do this, we are contacting people and groups located within Arizona to convey that this project will directly impact the amphibian biodiversity of our state and in other parts of the world.

How do you keep the audience engaged over time?

We’ve created a Facebook group (https://www.facebook.com/groups/905234229573944/) to keep our audience up to date on the progression of our research project as it’s being conducted. This will keep our audience up to date on the current status of our project and the exciting discoveries we’re making. In addition to our Facebook group, we are also planning to present the results of our study in a scientific journal article and present this information to our community in a series of talks.

What can we do to inspire the next generation to want to help the environment and the wildlife?

The information we gain from completing this study will directly lend to the construction of effective amphibian biodiversity conservation strategies. Without the help from our next generation we are at risk of losing much of the amazing amphibian biodiversity on our planet. The Frogs Are Green organization could be a wonderful partner in helping us to spread our project to its supporters. We feel that this organization can help reach a younger and broader audience than we’ve been able to reach thus far. In order for the meaningfulness of our research to be effective we need our next generations to know about the wonderful and beautiful amphibian biodiversity our planet has to offer.

What can people do to help? Donate and/or share to your cause/work?

We would be most appreciative if individuals would pledge to share our campaign with friends and colleagues using social media. We hope to reach as many people as possible because we feel the current state of our planet’s amphibian biodiversity is at a critical point. As a community we need to act now to preserve these species to maintain healthy ecosystems and for future generations. Sharing our campaign would help us reach our goal however we would be extremely thankful if people would also donate to our project so we can conduct this extremely important research.

10/20/15

Frog color patterns and the lack of color on the ventral surface

Frogs are a component of an exclusive cluster of the animal realm that have a part in the subtle equilibrium of both the ecosystem and the food chain. Frogs can be seen more or less any place apart from Antarctica. The majority of frogs are found in tropical areas and more frogs are found in the hotter countries. There are approximately 4,740 species of frogs on the planet. They are in fact remarkable creatures that come in a huge range of sizes as well as colors.

Frogs generally eat insects such as flies, as well as, worms and small fish. In some cultures, frogs are believed to bring good luck. Noticeably, some frogs, such as the poison dart frog, have an adequate amount of toxin in their miniscule bodies to take the life of a human being. Some species of frogs are capable of changing their skin color, and a few of them have a similar skin color as their environment.

By liz west (leopard frog2) [CC BY 2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

Without a doubt, frogs have several natural predators. Generally frogs are responsive to predators, which is why frogs put down a lot of eggs at a time. A few of the main predators of frogs are comprised of reptiles, like snakes and lizards. Some fish will eat frogs, and birds will also eat frogs. Frogs are always in danger of predators and unfortunately, humans also eat frogs. Luckily, frogs have developed many techniques for defending themselves from these predators.

The color patterns of frogs and their lack of color on the ventral surface, allow frogs to escape from predators. Usually, the underside of the frog is a lighter color than the top side for the reason that if the frog is hanging on top of the water and a predator is searching for a frog, the suns glare makes the frog difficult to spot. There are shady marks on the bottom and as a result it doesn’t expose the silhouette of the frog. Some of the frog’s upper side is darker since when swimming in the underneath of a dark pond, so it coordinates with the bottom.

By fa:User:Juybari (fa:File:Frog in Water.jpg) [GFDL (http://www.gnu.org/copyleft/fdl.html) or CC-BY-SA-3.0 (http://creativecommons.org/licenses/by-sa/3.0/)], via Wikimedia Commons

Frogs have a huge range of skin colors and patterns, which indeed help them from their natural predators. Colors can aid as a warning to predators that the frog may be toxic. Some frogs have the ability to change the color of their skin to adjust their heat soaking up rate, which assist them in managing their temperature. Just like other creatures, a frog’s skin and its color can be a sign of poison. Eating a blue frog can be deadly. So, the blue colored frogs offer a sign that they are not edible.

By Michael Gäbler (own work (eigenes Werk)) [CC BY 3.0 (http://creativecommons.org/licenses/by/3.0)], via Wikimedia Commons

Some studies have shown that the techniques for getting the better of a predator rely on the species of frog, but several of these resistances contain the utilization of color. It is found that some of the frogs have developed patterns on their backs that bamboozle or confuse aerial predators. The patterns cover up the shape of the frog; as a result, the predator doesn’t identify it as something safe to eat.

The largest parts of frogs are not dangerous, but there are some frogs that take advantage of poison as a self-protection tactic. Research reveals that some frogs have deadly poisons that could make a human harshly sick, or even kill someone. The toxic frogs such as the poison dart frog generally have brightly colored skin that stands as a caution. The toxic frogs have in fact very few predators. Frogs such as the barred leaf frog have light patterns on their legs and body. So, when the frog runs, these patterns will make the predator puzzled.

The animal kingdom makes use of a lot of tactics in order to save themselves from predators. Frogs are amazing and they use a lot of techniques to fool predators. Basically the upper surfaces of frogs are dark and go with their environment so that they are hidden from predators viewing them from above. The ventral surfaces of most of the frogs are normally a light color so that it will be disguised against the lighter sky while observed from underneath.

On land, a frog’s enemies will attack them from above and therefore, the color on its upper side serves as concealment. In water, the frog is susceptible to assault from below. For the most part, frog’s ventral surface is seldom uncovered to the sight of predators, so these surfaces don’t require camouflage. Frogs have a variety of patterns and colors that protects them from natural predators and harsh environments.

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Guest blog by Ligia Blake, who is a freelance blog writer and works for essayscouncil.com, a custom essay writing service with a passion of helping out students.

08/3/15

Will the Fungus Killing Salamanders Come to the US?

There is nothing more important right now to the survival of Salamanders than the United States stopping the importation of them from Europe and Asia.

From the New York Times article, “Importing Both Salamanders and Their Potential Destruction.”

“You would think there would be something in place,” said Vance T. Vredenburg, a biologist at San Francisco State University. “We really need a government agency at some level to take action and do something.”

…The pet trade brings huge numbers of salamanders into the United States. Fish and Wildlife Service records show that about 780,000 salamanders were imported from 2010 to 2014. Dr. Vredenburg and his colleagues found that 98 percent of the imported animals were native to Asia.

We have been watching Chytrid Fungus, or Batrachochytrium dendrobatidis (Bd for short) wipe out many species of frogs. Humans have helped spread this disease around the world and now we have an opportunity, if we act quickly to help the salamanders.

We’re worried about the Asian fungus that causes the disease “Bsal” which has already reached Europe, wiping out 96 percent of fire salamanders in the Netherlands. Now, researchers have determined that the fungus will spread like wildfire if it reaches North America, and they’re calling for an immediate ban on all salamander imports.

Fire Salamander from Wikipedia Commons.

Below are a series of articles on this topic from the past week:

NPR >> Scientists Urge Ban On Salamander Imports To U.S. To Keep Fungus At Bay

UC Berkeley >> Scientists urge ban on salamander imports to fend off new fungus

The Verge >> North America’s salamanders threatened by bloody skin disease

Tech Times >> Bsal Fungus Can Wipe Out U.S. Salamander Population: What To Know

06/4/15

DAYS OF MADAGASCAR 2015

GIORNATE DEL MADAGASCAR 2015 / DAYS OF MADAGASCAR 2015
The island of Marco Polo

June 12 and 13, 2015
Venice, Museum of Natural History

Isolated from Africa to many tens of millions of years, Madagascar has developed its own peculiar fauna and flora, dramatically different from that of other land masses, near and far.

Similarly colonization by man, which took place on a massive scale only for two thousand years, has seen the mix of elements Africans, Asians, Arabs and Europeans who have forged a culture of “metissage” composed of no less than 18 ethnic groups each with its particular history and traditions, have in common the basic language of Indonesian origin and the cult of the dead, called “famadihana”.

Unfortunately Madagascar is also a land of great contrasts, with widespread problems of social and economic. The days that pay special attention to aspects concerning the natural wealth and cultural diversity of this island, home to the intervention of researchers that deal with biodiversity and personnel working in health, showing how much Italy is engaged in this country.

In collaboration with the Regional Museum of Natural Sciences (Museo Regionale di Scienze Naturali) of Turin and the Association “Malagasy Miray.”

Video below: Interview of Franco Andreone (herpetologist) at Andriamanero, Isalo National Park.

This video is in Italian: #madagascarexpedition2013: Betampona Rainforest

DOWNLOAD THE PRESENTATION OF THE INITIATIVE (.ppt 12.3 MB) >>>

PROGRAM

Friday, June 12, 2015 20.30 – Cinema Giorgione

Screening of the film in English “Island of lemurs in Madagascar” by David Douglas and Drew Fellman, with narrated by Morgan Freeman and with Patricia Wright

Introduction and presentation of: Franco Andreone (Museo Regionale di Scienze Naturali Regional Museum of Natural Sciences of Turin) Giuseppe Donati (Oxford Brookes University)

Entrance to the Cinema Giorgione free until all available seats

Saturday, June 13, 2015 – Natural History Museum

10.30 Welcome and opening of the day
Gabriella Belli (Director Civic Museums Foundation of Venice)
Paola Casagrande (Director of the Museo Regionale di Scienze Naturali Regional Museum of Natural Sciences of Turin) Randrianantoandro Solofo Theophile (Minister Counsellor Embassy of Madagascar)

Franco Andreone (Museo Regionale di Scienze Naturali Regional Museum of Natural Sciences of Turin)
Madagascar: stories from a biodiverse land biodiverse

Giuseppe Donati (Oxford Brookes University)
Survive the next day: the lesson of lemurs

15:00

Riccardo Bononi (IRFOSS Padua)
Life, death and disease in the ancestor worship

Italian volunteers in Madagascar
Friends Amici di Jangany
The Italian volunteer in MadagascarVolontari Italiani in Madagascar

Olga del Madagascar
Culture, nature and music: songs taken from ‘album “Ma nature”

Tasting The with Malagasy vanilla

Hours 10:00 to 18:00 – Gallery of Cetaceans

Photo exhibition “Madagasikara” by Franco Andreone: throughout the day and until August 2, 2015 will be exhibited suggestive images dedicated to the nature, history and traditions of Madagascar.

Information points: voluntary associations will be on hand to talk about their experience in Madagascar

WORKSHOPS:
appointment until all available seats

Hours 10:30 to 12:00 and 15:30 to 17:00
Children aged 7 to 11 years

“The nature of the island”, edited by Coop. Silty
“Sounds and rhythms of Madagascar”, edited by Olga del Madagascar:

10:15, 11:30, 15:00, 16:15
For children 4 to 6 years accompanied by their parents

“The chameleon says narrates, animal stories and legends of Madagascar” by Barchetta Blu

INFORMATION AND RESERVATIONS:

The day is free entry until all the places available, except for laboratories that require an admission ticket to the museum (free for residents and people born in Venice, upon presentation of a photo ID).

To book workshops call 041 2750206

The photo exhibition will be open for free only on the occasion of this day and until August 2, 2015 is required to be in possession of a ticket to the museum.

Information shared by:

Franco Andreone
Museo Regionale di Scienze Naturali
Via G. Giolitti, 36
I-10123 TORINO – ITALY
website www.francoandreone.it
Facebook www.facebook.com/franco.andreone
Twitter @francoandreone
Youtube Betampona
Youtube Isalo

02/27/15

Chytrid Fungus is Found in Madagascar

The entire amphibian class is currently afflicted by a global pandemic that is accelerating extinction at an alarming rate. Until now, a few islands like Madagascar were thought not to have been affected. However, an analysis of the latest series of tests shows that the chytrid fungus also poses a threat to amphibians in Madagascar. “This is sad news for herpetologists around the world,” says Dr. Dirk Schmeller of the UFZ, who was involved in analyzing the samples and has, together with Elodie Courtois, detected Bd in samples from Madagascar collected in 2010. “Firstly, it means that an island that is home to a particularly high number of amphibian species is now at risk. Several hundred species live only on this island. And, secondly, if the pathogen has managed to reach such a secluded island, it can and will occur everywhere.”

IMAGE: Chytrid fungus was proved on Platypelis pollicaris from Ranomafana. view more
Credit: Miguel Vences / TU Braunschweig

Prof. Miguel Vences from TU Braunschweig adds, “The chytrid fungus was found in all four families of the indigenous Madagascan frogs, which means it has the potential to infect diverse species. This is a shock!” The study also shows that the disease affects amphibians at medium to high altitudes, which ties in with observations from other parts of the world, where the effects of the amphibian epidemic have been felt primarily in the mountains.

“Luckily, there have not yet been any dramatic declines in amphibian populations in Madagascar,” Dirk Schmeller reports. “However, the pathogen appears to be more widespread in some places than others. Madagascar may have several strains of the pathogen, maybe even the global, hypervirulent strain. This shows how important it is to be able to isolate the pathogen and analyze it genetically, which is something we haven’t yet succeeded in doing.” At the same time, the researchers recommend continuing with the monitoring program across the entire country to observe the spread of the disease. The scientists also suggest setting up extra breeding stations for key species, in addition to the two centers already being built, to act as arks, so that enough amphibians could be bred to recolonize the habitats in a crisis. “We are also hopeful that we may be able to suppress the growth of the Bd pathogen with the help of skin bacteria,” says Miguel Vences. “It might then be possible to use these bacteria as a kind of probiotic skin ointment in the future.” A high diversity of microbial communities in the water could also reduce the potential for infection, according to earlier investigations conducted by Dirk S. Schmeller, Frank Pasmans, and their teams (published in Current Biology).

The outbreak of amphibian chytridiomycosis in Madagascar puts an additional seven per cent of the world’s amphibian species at risk, according to figures from the Amphibian Survival Alliance (ASA). “The decline in Madagascan amphibians is not just a concern for herpetologists and frog researchers,” says Dr. Franco Andreone, “It would be a great loss for the entire world.”

Guest post by Dr. Dirk Schmeller
Originally published:
http://www.eurekalert.org/pub_releases/2015-02/hcfe-acf022615.php

02/8/15

Frogs and Water Quality

Yesterday, in class with the Cloud Institute and Sustainable Jersey (NJ Learns), each student got to briefly share their project, so I was describing the Frogs Are Green mission and projects on the agenda for 2015.

My teacher Jaimie P. Cloud, challenged me with this question. “Much of what Frogs Are Green is about is bringing awareness to the public, but is there a change I want in the public’s behavior?”

It made me think about one specific area that we can focus on which would help frogs and amphibians as well as other wildlife and humans, and that is water quality.

We know that there are many issues, such as, pollution and oil spills, pesticides and other toxins, pharmaceuticals, and these aren’t just affecting wildlife, but us as well. It’s humans that are creating many of these problems, so it’s obvious that we must be the ones to correct it.

I did a search on Google for Frogs and Water Quality and was surprised to learn there is currently no standard for water quality as it applies to frogs and amphibians:
“The factors thought to be contributing to declines in frog populations include habitat loss, introduction of exotic species, overexploitation, disease, climate change, and decreasing water quality. With respect to water quality, agroecosystems use 80-90% of the water resources in the western United States, frequently resulting in highly eutrophic conditions. Recent investigations suggest that these eutrophic conditions (elevated pH, water temperature, and un-ionized ammonia) may be associated with frog embryo mortality or malformations. However, water quality criteria for frogs and other amphibians do not currently exist. Here, we briefly review data that support the need to develop water quality parameters for frogs in agroecosystems and other habitats.”
(from this website: PMC1519110/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1519110/)

What this tells me is that currently the water quality is tested to make sure it’s safe for humans, but there are no regulations in place for it to be safe for wildlife? Doesn’t it matter that it’s healthy for all life?

I’ll be reading much more on this issue and would like to invite you to share your thoughts or links to additional documentation you may have found.

Here’s a video I’d like to share because it shows just how important it is to educate the young with hands-on education from Marie Hartford, Science, 5th Grade Teacher and her students in Redmond, WA on the Teaching Channel about “Measuring the pH as it Relates to Water Quality.”

Frogs Are Green

Environmental Organization Helping to Bring Awareness About Frog Decline Around The World

Category Archives: Amphibian research